Magnetophoretic Position Detection for Multiplexed Immunoassays Using Colored Microspheres
نویسندگان
چکیده
This paper presents a new magnetophoretic position detection method for multiplexed immunoassay using colored microspheres for encoding of target analytes and superparamagnetic nanoparticles (SMNPs) for quantitative analysis. Under the magnetic field gradient, reacted microspheres are deflected as much as analytes and SMNPs are conjugated with them. The positions of colored microspheres were correlated with the concentration of each analyte. Three immunoglobuline Gs (IgGs) were used as a model analyte. Detection limits of goat, rabbit and mouse IgG-biotins were estimated to be 10.9, 30.6 and 12.1 fM, respectively. By adjusting flow rate or detection zone, the dynamic range could be controlled.
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